Three commercial sandwich enzyme-linked immunosorbent assay (ELISA) test kits for the detection of hazelnuts and almonds were evaluated. Limits of detection and dynamic ranges were determined for hazelnuts and almonds spiked into cooked oatmeal, dipping chocolate, and muffins (baked).
The limit of detection values varied from 1 to 38 μg/g, depending on the food matrix and ELISA test kit. Percent recoveries based on the standards supplied with the test kits varied from 10% to 170%. It is impossible to ascertain whether the percent recoveries reflect the performance of the ELISAs or differences between the protein content of the nuts used to spike the samples and the test kit standards.
Unfortunately, reference materials do not exist that can be used to compare the results from different test kits and standardize the test kit standards. Also, insufficient knowledge regarding the epitope specificity of the antibodies used in the ELISAs further hinders interpretation of the results generated by the different test kits.
[Improved capability of a revised test kit, FASTKIT ELISA version II (Egg), to detect heated proteins of egg white but not egg yolk].
BACKGROUND
A kit, FASTKIT ELISA version II (Egg) (Nippon Meat Packers) is an enzyme-linked immunosorbent assay kit for detecting hen’s egg proteins in foodstuffs. This kit is an enhanced version of FASTKIT ELISA (Egg) with a greater efficiency in terms of extraction of egg proteins from heated foodstuffs. However, the property of this kit remains to be fully elucidated.
Annexin V- FITC |
|||
| E16FXP018a-100T | EnoGene | 100 tests | 400 EUR |
|
Description: Available in various conjugation types. |
|||
Annexin V FITC |
|||
| EXB0024 | ExBio | 100 tests | 165 EUR |
Annexin V FITC |
|||
| MBS1802116-100Tests | MyBiosource | 100Tests | 280 EUR |
Annexin V FITC |
|||
| MBS1802116-5x100Tests | MyBiosource | 5x100Tests | 1020 EUR |
Annexin V, FITC Labeled |
|||
| 20030 | AAT Bioquest | 100 tests | 227 EUR |
|
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
|||
Annexin V, FITC Labeled |
|||
| 20030-100tests | AAT Bioquest | 100 tests | 222 EUR |
|
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. |
|||
Annexin V, FITC Conjugate |
|||
| 29001 | Biotium | 0.5ML | 274 EUR |
|
Description: BSA from bovine serum (cow; Bos taurus), Annexin V (recombinant produced in E. coli) |
|||
Annexin V, FITC Conjugate |
|||
| 29001-1 | Biotium | EA | 274 EUR |
Annexin V FITC (500 tests) |
|||
| EXB0029 | ExBio | 500tests | 577.5 EUR |
ANNEXIN V: FITC ASSAY KIT |
|||
| GWB-Q00433 | GenWay Biotech | 100 TESTS | Ask for price |
ANNEXIN V: FITC ASSAY KIT |
|||
| GWB-Q00434 | GenWay Biotech | 300 TESTS | Ask for price |
Annexin V Antibody (FITC) |
|||
| GWB-22DB78 | GenWay Biotech | 100 TESTS | Ask for price |
Annexin V Solution (FITC) |
|||
| abx095272-100l | Abbexa | 100 µl | 300 EUR |
Annexin V Solution (FITC) |
|||
| abx095272-1ml | Abbexa | 1 ml | Ask for price |
Annexin V Solution (FITC) |
|||
| abx095272-200l | Abbexa | 200 µl | 800 EUR |
OOTA00688-25T - Annexin V : FITC |
|||
| OOTA00688-25T | Aviva Systems Biology | 25Tests | 79 EUR |
OOTA00688-100T - Annexin V : FITC |
|||
| OOTA00688-100T | Aviva Systems Biology | 100Tests | 209 EUR |
Annexin V (ANXA5) Antibody (FITC) |
|||
| abx414513-100tests | Abbexa | 100 tests | 610.8 EUR |
Annexin V (human), (recombinant) (FITC) |
|||
| MBS566110-100Tests | MyBiosource | 100Tests | 325 EUR |
Annexin V (human), (recombinant) (FITC) |
|||
| MBS566110-3x100Tests | MyBiosource | 3x100Tests | 650 EUR |
Annexin V FITC Apoptosis Detection Kit |
|||
| MBS697178-1Kit | MyBiosource | 1Kit | 335 EUR |
Annexin V FITC Apoptosis Detection Kit |
|||
| MBS697178-5x1Kit | MyBiosource | 5x1Kit | 1475 EUR |
OPSB00055-100T - Aposcreen Annexin V : FITC |
|||
| OPSB00055-100T | Aviva Systems Biology | 100Tests | 269 EUR |
Annexin V-FITC |
|||
| MBS2557006-100Tests | MyBiosource | 100Tests | 200 EUR |
Annexin V-FITC |
|||
| MBS2557006-200Tests | MyBiosource | 200Tests | 270 EUR |
Annexin V-FITC |
|||
| MBS2557006-500Tests | MyBiosource | 500Tests | 530 EUR |
Annexin V-FITC |
|||
| MBS2557006-50Tests | MyBiosource | 50Tests | 155 EUR |
Annexin V-FITC |
|||
| MBS2557006-5x500Tests | MyBiosource | 5x500Tests | 2375 EUR |
Annexin V-FITC |
|||
| MBS355209-100Tests | MyBiosource | 100Tests | 370 EUR |
Annexin V-FITC |
|||
| MBS355209-25Tests | MyBiosource | 25Tests | 250 EUR |
Annexin V (FITC) / PI Apoptosis Detection Kit |
|||
| abx090601-100tests | Abbexa | 100 tests | 552 EUR |
Annexin V Apoptosis Detection Kit (FITC) |
|||
| abx290002-100test | Abbexa | 100 test | 644.4 EUR |
Annexin V Apoptosis Detection Kit (FITC) |
|||
| abx290014-200test | Abbexa | 200 test | 577.2 EUR |
Annexin V Apoptosis Detection Kit (FITC) |
|||
| 55R-1265 | Fitzgerald | 25 assays | 567 EUR |
|
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory |
|||
Annexin V Polyclonal Antibody FITC Conjugated |
|||
| MBS9462180-01mL | MyBiosource | 0.1mL | 595 EUR |
Annexin V Polyclonal Antibody FITC Conjugated |
|||
| MBS9462180-5x01mL | MyBiosource | 5x0.1mL | 2525 EUR |
Annexin V (FITC) / 7-AAD Apoptosis Detection Kit |
|||
| abx090603-100tests | Abbexa | 100 tests | 594 EUR |
Annexin V / PI Apoptosis Detection Kit (FITC) |
|||
| abx290007-100test | Abbexa | 100 test | 543.6 EUR |
Annexin V-FITC Reagent |
|||
| 1001-1000 | Biovision | each | 1495.2 EUR |
Annexin V-FITC Reagent |
|||
| 1001-200 | Biovision | each | 529.2 EUR |
Annexin V-FITC Reagent |
|||
| E-CK-A111-200Tests | Elabscience Biotech | 200 Tests | 298 EUR |
Annexin V-FITC Reagent |
|||
| E-CK-A111-500Tests | Elabscience Biotech | 500 Tests | 660 EUR |
Annexin V-FITC Reagent |
|||
| E-CK-A111-50Tests | Elabscience Biotech | 50 Tests | 110 EUR |
Annexin V-FITC Reagent |
|||
| MBS355858-100Tests | MyBiosource | 100Tests | 370 EUR |
Annexin V-FITC Reagent |
|||
| MBS355858-5x100Tests | MyBiosource | 5x100Tests | 1370 EUR |
Annexin V-FITC Reagent |
|||
| E-CK-A111-100Tests | Elabscience Biotech | 100 Tests | 190 EUR |
|
Description: Apoptosis/Annexin V |
|||
Annexin V / 7ADD Apoptosis Detection Kit (FITC) |
|||
| abx290008-100test | Abbexa | 100 test | 543.6 EUR |
Annexin V Apoptosis Detection Kit Plus (FITC) |
|||
| 55R-1302 | Fitzgerald | 25 assays | 547 EUR |
|
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory |
|||
Aposcreen Annexin V-FITC |
|||
| MBS670379-100Tests | MyBiosource | 100Tests | 245 EUR |
Aposcreen Annexin V-FITC |
|||
| MBS670379-5x100Tests | MyBiosource | 5x100Tests | 955 EUR |
Aposcreen Annexin V-FITC |
|||
| MBS670381-100Tests | MyBiosource | 100Tests | 310 EUR |
Aposcreen Annexin V-FITC |
|||
| MBS670381-5x100Tests | MyBiosource | 5x100Tests | 1240 EUR |
Aposcreen Annexin V Apoptosis Kit : FITC (OKSB00007) |
|||
| OKSB00007 | Aviva Systems Biology | 100 Tests | 615.6 EUR |
|
Description: Description of target: ;Species reactivity: ;Application: FC, ICC;Assay info: ;Sensitivity: |
|||
Annexin V Apoptosis Detection Kit : FITC (OKTA00003) |
|||
| OKTA00003 | Aviva Systems Biology | KIT | 380.4 EUR |
|
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
|||
OPSB00049-100T - Aposcreen Annexin V Protein : FITC |
|||
| OPSB00049-100T | Aviva Systems Biology | 100Tests | 189 EUR |
ANXA10, CT (ANXA10, ANX14, Annexin A10, Annexin-10, Annexin-14) (FITC) |
|||
| MBS6273920-02mL | MyBiosource | 0.2mL | 980 EUR |
ANXA10, CT (ANXA10, ANX14, Annexin A10, Annexin-10, Annexin-14) (FITC) |
|||
| MBS6273920-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
Annexin A13 (ANXA13, ISA, ANX13, Annexin XIII, Annexin-13, Intestine-specific Annexin) (FITC) |
|||
| MBS6399528-01mL | MyBiosource | 0.1mL | 1040 EUR |
Annexin A13 (ANXA13, ISA, ANX13, Annexin XIII, Annexin-13, Intestine-specific Annexin) (FITC) |
|||
| MBS6399528-5x01mL | MyBiosource | 5x0.1mL | 4540 EUR |
Annexin A13 (ANXA13, ISA, ANX13, Annexin XIII, Annexin-13, Intestine-specific Annexin) (FITC) |
|||
| MBS6399539-01mL | MyBiosource | 0.1mL | 1040 EUR |
Annexin A13 (ANXA13, ISA, ANX13, Annexin XIII, Annexin-13, Intestine-specific Annexin) (FITC) |
|||
| MBS6399539-5x01mL | MyBiosource | 5x0.1mL | 4540 EUR |
ANXA9, ID (ANXA9, ANX31, Annexin A9, Annexin XXXI, Annexin-31, Annexin-9, Pemphaxin) (FITC) |
|||
| MBS6273964-02mL | MyBiosource | 0.2mL | 980 EUR |
ANXA9, ID (ANXA9, ANX31, Annexin A9, Annexin XXXI, Annexin-31, Annexin-9, Pemphaxin) (FITC) |
|||
| MBS6273964-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
Annexin V (human), (recombinant) (FITC) (Content: 100 tests) |
|||
| MBS566107-1Vial | MyBiosource | 1Vial | 625 EUR |
Annexin V (human), (recombinant) (FITC) (Content: 100 tests) |
|||
| MBS566107-5x1Vial | MyBiosource | 5x1Vial | 3995 EUR |
FITC Linked Monoclonal Antibody to Annexin V (ANXA5) |
|||
| MBS2129057-INQUIRE | MyBiosource | INQUIRE | Ask for price |
ANXA10 (Annexin-10, Annexin-14, ANXA10, ANX14) (FITC) |
|||
| MBS6369905-01mL | MyBiosource | 0.1mL | 920 EUR |
ANXA10 (Annexin-10, Annexin-14, ANXA10, ANX14) (FITC) |
|||
| MBS6369905-5x01mL | MyBiosource | 5x0.1mL | 3990 EUR |
ANXA10 (Annexin-10, Annexin-14, ANXA10, ANX14) (FITC) |
|||
| MBS6145913-01mL | MyBiosource | 0.1(mL | 875 EUR |
ANXA10 (Annexin-10, Annexin-14, ANXA10, ANX14) (FITC) |
|||
| MBS6145913-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
ANXA13, NT (ANXA13, ANX13, Annexin A13, Annexin XIII, Annexin-13, Intestine-specific annexin) (FITC) |
|||
| MBS6273931-02mL | MyBiosource | 0.2mL | 980 EUR |
ANXA13, NT (ANXA13, ANX13, Annexin A13, Annexin XIII, Annexin-13, Intestine-specific annexin) (FITC) |
|||
| MBS6273931-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
Annexin V-FITC Apoptosis Kit |
|||
| K2003-100 | ApexBio | 100 assays | 354 EUR |
|
Description: Detects Apoptosis & Necrosis within 10 min. |
|||
Annexin V-FITC Apoptosis Kit |
|||
| K2003-25 | ApexBio | 25 assays | 143 EUR |
|
Description: Detects Apoptosis & Necrosis within 10 min. |
|||
Annexin V-FITC Apoptosis Kit |
|||
| K2003-400 | ApexBio | 400 assays | 810 EUR |
|
Description: Detects Apoptosis & Necrosis within 10 min. |
|||
Annexin V-FITC Apoptosis Kit |
|||
| K101-100 | Biovision | each | 614.4 EUR |
Annexin V-FITC Apoptosis Kit |
|||
| K101-25 | Biovision | each | 268.8 EUR |
Annexin V-FITC Apoptosis Kit |
|||
| K101-400 | Biovision | each | 1227.6 EUR |
Annexin V-FITC Apoptosis Kit |
|||
| MBS7608274-INQUIRE | MyBiosource | INQUIRE | Ask for price |
FITC-Linked Monoclonal Antibody to Annexin V (ANXA5) |
|||
| LAA259Hu82 | Cloud-Clone | 119 ul | 329 EUR |
Annexin A5 (Annexin V)-Biotin |
|||
| MC-252 | Kamiya Biomedical Company | VAA-33 | Ask for price |
OKSB00007-100T - Aposcreen Annexin V Apoptosis Kit : FITC |
|||
| OKSB00007-100T | Aviva Systems Biology | 100Tests | 399 EUR |
OKTA00003-1KIT - Annexin V Apoptosis Detection Kit : FITC |
|||
| OKTA00003-1KIT | Aviva Systems Biology | KIT | 269 EUR |
Annexin V |
|||
| E8ET1702-62 | EnoGene | 100ul | 275 EUR |
|
Description: Available in various conjugation types. |
|||
Annexin V |
|||
| Annv | Clemente Associates LLC | 2 ML | 367.5 EUR |
Annexin V |
|||
| ANXVCFB-200T | ImmunoStep | 200 test | 350.9 EUR |
|
Description: Annexin V conjugate in the selected fluorochrome |
|||
Annexin V |
|||
| ANXVDY-200T | ImmunoStep | 200 test | 287.1 EUR |
|
Description: Annexin V conjugate in the selected fluorochrome |
|||
Annexin V |
|||
| ANXVF-200T | ImmunoStep | 200 test | 259.6 EUR |
|
Description: Annexin V conjugate in the selected fluorochrome |
|||
Annexin V |
|||
| ANXVPE-200T | ImmunoStep | 200 test | 287.1 EUR |
|
Description: Annexin V conjugate in the selected fluorochrome |
|||
Annexin V |
|||
| abx095275-100l | Abbexa | 100 µl | 443.75 EUR |
Annexin V |
|||
| abx095275-1ml | Abbexa | 1 ml | Ask for price |
Annexin V |
|||
| abx095275-200l | Abbexa | 200 µl | Ask for price |
Annexin V |
|||
| MBS8534369-01mL | MyBiosource | 0.1mL | 345 EUR |
Annexin V |
|||
| MBS8534369-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
Annexin V |
|||
| MBS8534369-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
Annexin V |
|||
| MBS8534369-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
Annexin V |
|||
| MBS8534369-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
Annexin V |
|||
| MBS1802274-01mg | MyBiosource | 0.1mg | 305 EUR |
Annexin V |
|||
| MBS1802274-5x01mg | MyBiosource | 5x0.1mg | 1120 EUR |
Annexin V 647 |
|||
| E16FXP023a-100T | EnoGene | 100 tests | 433.33 EUR |
|
Description: Available in various conjugation types. |
|||
Annexin A5/Annexin V Rabbit mAb |
|||
| A9262 | Abclonal | 200μL | 213.2 EUR |
|
Description: Recombinant fusion protein containing a sequence corresponding to amino acids 165-225 of human CD79a (NP_001774.1). |
|||
Annexin A5/Annexin V Rabbit pAb |
|||
| A13945 | Abclonal | 200μL | 1246.96 EUR |
Annexin A5 (Annexin V) (VAC-alpha) |
|||
| MC-250 | Kamiya Biomedical Company | VAA-33 | Ask for price |
Annexin V-FITC/PI Apoptosis Kit |
|||
| E-CK-A211-100Assays | Elabscience Biotech | 100 Assays | 260 EUR |
Annexin V-FITC/PI Apoptosis Kit |
|||
| E-CK-A211-200Assays | Elabscience Biotech | 200 Assays | 398 EUR |
Annexin V-FITC/PI Apoptosis Kit |
|||
| E-CK-A211-20Assays | Elabscience Biotech | 20 Assays | 75 EUR |
Annexin V-FITC/PI Apoptosis Kit |
|||
| K2003-20 | ApexBio | 20assays | 56 EUR |
|
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits |
|||
Annexin V-FITC/PI Apoptosis Kit |
|||
| K2003-50 | ApexBio | 50assays | 112 EUR |
|
Description: Apoptosis Kit|Apoptosis Detection kit#Kits|Apoptosis Kit#Kits |
|||
Annexin V-FITC/PI Apoptosis Kit |
|||
| E-CK-A211-50Assays | Elabscience Biotech | 50 Assays | 150 EUR |
|
Description: Apoptosis/Annexin V |
|||
Annexin A2 antibody (FITC) |
|||
| 60R-1336 | Fitzgerald | 100 ug | 302 EUR |
|
Description: Rabbit polyclonal Annexin A2 antibody (FITC) |
|||
Annexin A5 antibody (FITC) |
|||
| 60R-1514 | Fitzgerald | 100 ug | 302 EUR |
|
Description: Rabbit polyclonal Annexin A5 antibody (FITC) |
|||
Annexin A5 Antibody (FITC) |
|||
| 20-abx300581 | Abbexa |
|
|
Annexin A2 antibody (FITC) |
|||
| MBS5310205-01mg | MyBiosource | 0.1mg | 510 EUR |
Annexin A2 antibody (FITC) |
|||
| MBS5310205-5x01mg | MyBiosource | 5x0.1mg | 2150 EUR |
ANXA7 (Synexin, Annexin VII, Annexin-7, Annexin A7, ANX7, SNX, OK/SW-cl.95) (FITC) |
|||
| MBS6399660-01mL | MyBiosource | 0.1mL | 1040 EUR |
ANXA7 (Synexin, Annexin VII, Annexin-7, Annexin A7, ANX7, SNX, OK/SW-cl.95) (FITC) |
|||
| MBS6399660-5x01mL | MyBiosource | 5x0.1mL | 4540 EUR |
ANXA7 (Synexin, Annexin VII, Annexin-7, Annexin A7, ANX7, SNX, OK/SW-cl.95) (FITC) |
|||
| MBS6399671-01mL | MyBiosource | 0.1mL | 1040 EUR |
ANXA7 (Synexin, Annexin VII, Annexin-7, Annexin A7, ANX7, SNX, OK/SW-cl.95) (FITC) |
|||
| MBS6399671-5x01mL | MyBiosource | 5x0.1mL | 4540 EUR |
Annexin V-FITC/DAPI Apoptosis Kit |
|||
| E-CK-A252-100Assays | Elabscience Biotech | 100 Assays | 260 EUR |
Annexin V-FITC/DAPI Apoptosis Kit |
|||
| E-CK-A252-200Assays | Elabscience Biotech | 200 Assays | 398 EUR |
Annexin V-FITC/DAPI Apoptosis Kit |
|||
| E-CK-A252-20Assays | Elabscience Biotech | 20 Assays | 75 EUR |
Annexin V-FITC/DAPI Apoptosis Kit |
|||
| E-CK-A252-50Assays | Elabscience Biotech | 50 Assays | 150 EUR |
Annexin V-FITC Apoptosis Kit Plus |
|||
| K201-100 | Biovision | each | 601.2 EUR |
Annexin V-FITC Apoptosis Kit Plus |
|||
| K201-25 | Biovision | each | 288 EUR |
Annexin V-FITC Apoptosis Kit Plus |
|||
| K201-400 | Biovision | each | 1136.4 EUR |
Annexin V-FITC Apoptosis Kit Plus |
|||
| K2057-100 | ApexBio | 100 assays | 619.2 EUR |
Annexin V-FITC Apoptosis Kit Plus |
|||
| K2057-25 | ApexBio | 25 assays | 202 EUR |
|
Description: Detects Apoptotic, Necrotic & Healthy cells within 10 min |
|||
Annexin V-FITC Apoptosis Kit Plus |
|||
| K2057-400 | ApexBio | 400 assays | 1270.8 EUR |
ANXA11 (Annexin A11, Annexin-11, Annexin XI, Calcyclin-associated Annexin 50, CAP-50, 56kD Autoantigen, ANX11) (FITC) |
|||
| MBS6399561-01mL | MyBiosource | 0.1mL | 1040 EUR |
ANXA11 (Annexin A11, Annexin-11, Annexin XI, Calcyclin-associated Annexin 50, CAP-50, 56kD Autoantigen, ANX11) (FITC) |
|||
| MBS6399561-5x01mL | MyBiosource | 5x0.1mL | 4540 EUR |
ANXA7, ID (Synexin, Annexin VII, Annexin-7, Annexin A7, ANX7, SNX, OK/SW-cl.95) (FITC) |
|||
| MBS6463211-02mL | MyBiosource | 0.2mL | 980 EUR |
ANXA7, ID (Synexin, Annexin VII, Annexin-7, Annexin A7, ANX7, SNX, OK/SW-cl.95) (FITC) |
|||
| MBS6463211-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
Annexin V (PE) |
|||
| abx090613-100tests | Abbexa | 100 tests | 644.4 EUR |
Annexin V PE |
|||
| EXB0027 | ExBio | 100 tests | 198 EUR |
Annexin V PE |
|||
| MBS697175-100Tests | MyBiosource | 100Tests | 300 EUR |
Annexin V PE |
|||
| MBS697175-25Tests | MyBiosource | 25Tests | 185 EUR |
Annexin V PE |
|||
| MBS697175-5x100Tests | MyBiosource | 5x100Tests | 1315 EUR |
Annexin V PE |
|||
| MBS1802118-100Tests | MyBiosource | 100Tests | 305 EUR |
Annexin V PE |
|||
| MBS1802118-5x100Tests | MyBiosource | 5x100Tests | 1120 EUR |
Annexin V (PE) |
|||
| MBS6251357-01mL | MyBiosource | 0.1(mL | 860 EUR |
Annexin V (PE) |
|||
| MBS6251357-5x01mL | MyBiosource | 5x0.1mL | 3720 EUR |
Annexin V (AP) |
|||
| MBS6247389-01mL | MyBiosource | 0.1(mL | 860 EUR |
Annexin V (AP) |
|||
| MBS6247389-5x01mL | MyBiosource | 5x0.1mL | 3720 EUR |
METHODS
Using this new kit, we measured the amount of egg proteins in unheated or heated (140 degrees C or 180 degrees C, 20 min) homemade cookies containing whole egg, egg white or egg yolk.
RESULTS
The capability for detection of unheated or heated (140 degrees C or 180 degrees C) whole egg proteins was similar. In addition, there was no significant difference in the detectability between heated (140 degrees C) whole egg and egg white proteins. However, unheated or heated (140 degrees C or 180 degrees C) egg yolk proteins were not sufficiently measured by this kit.
CONCLUSIONS
Our data suggest that this new kit is significantly improved for detection of heated egg white proteins as compared to that of old version, but not sufficient for detection of egg yolk proteins.