Evaluation of dengue NS1 antigen rapid tests and ELISA kits using clinical samples.
BACKGROUND
Early prognosis of dengue virus (DENV) an infection can enhance clinical outcomes by guaranteeing shut follow-up, initiating acceptable supportive therapies and elevating consciousness to the potential of hemorrhage or shock. Non-structural glycoprotein-1 (NS1) has confirmed to be a helpful biomarker for early prognosis of dengue.
A quantity of rapid diagnostic tests (RDTs) and enzyme-linked immunosorbent assays (ELISAs) concentrating on NS1 antigen (Ag) are actually commercially out there. Here we evaluated these tests using a well-characterized panel of clinical samples to find out their effectiveness for early prognosis.
RESULTS
Retrospective samples from South America had been used to judge the next tests: (i) “Dengue NS1 Ag STRIP” and (ii) “Platelia Dengue NS1 Ag ELISA” (Bio-Rad, France), (iii) “Dengue NS1 Detect Rapid Test (1st Generation)” and (iv) “DENV Detect NS1 ELISA” (InBios International, United States), (v) “Panbio Dengue Early Rapid (1st technology)” (vi) “Panbio Dengue Early ELISA (2nd technology)” and (vii) “SD Bioline Dengue NS1 Ag Rapid Test” (Alere, United States). Overall, the sensitivity of the RDTs ranged from 71.9%-79.1% whereas the sensitivity of the ELISAs different between 85.6-95.9%, using virus isolation because the reference technique. Most tests had decrease sensitivity for DENV-Four relative to the opposite three serotypes, had been much less delicate in detecting secondary infections, and seemed to be most delicate on Day 3-Four publish symptom onset. The specificity of all evaluated tests ranged from 95%-100%.
Anti GST tag Pab Rabbit |
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| 161025 | Antibody Research Corporation | 100 µg | Ask for price |
Anti GST tag Pab Chicken |
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| 111029 | Antibody Research Corporation | 100 µg | Ask for price |
GST -Tag |
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| 117217 | Antibody Research Corporation | 100 µg | Ask for price |
Anti Bovine Leptin Antibody |
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| 111002 | Antibody Research Corporation | 100 µg | Ask for price |
Anti Stercobilin Antibody Pab |
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| 165755 | Antibody Research Corporation | 100 µg | Ask for price |
Anti Plant Glycan Antibody [CCRC-M95] |
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| 401030 | Antibody Research Corporation | 100 µg | Ask for price |
Anti- GST Antibody |
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| 102-PA83 | ReliaTech | 50 µg | 89.25 EUR |
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Description: The anti-GST-tag antibody was generated by immunization of rabbits with a highly purified GST-tagged protein produced in E. coli. |
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Anti Mouse E3 Ubiquitin-Protein Ligase MYCBP2 Antibody, Phr1 |
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| 164056 | Antibody Research Corporation | 100 µg | Ask for price |
anti- GST Tag antibody |
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| FNab03683 | FN Test | 100µg | 606.3 EUR |
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Description: Antibody raised against GST Tag |
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anti- GST tag antibody |
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| FNab03684 | FN Test | 100µg | 606.3 EUR |
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Description: Antibody raised against GST tag |
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Anti- GST-tag Antibody |
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| GWB-2C28B7 | GenWay Biotech | 0.1 mg | Ask for price |
Anti-GST3/GST pi antibody |
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| MBS175754-01mg | MyBiosource | 0.1mg | 450 EUR |
Anti-GST3/GST pi antibody |
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| MBS175754-5x01mg | MyBiosource | 5x0.1mg | 1870 EUR |
Anti-GST3/GST pi Antibody |
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| MBS177460-01mg | MyBiosource | 0.1mg | 450 EUR |
Anti-GST3/GST pi Antibody |
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| MBS177460-5x01mg | MyBiosource | 5x0.1mg | 1870 EUR |
Anti-GST3/GST pi antibody |
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| MBS175164-01mg | MyBiosource | 0.1mg | 450 EUR |
Anti-GST3/GST pi antibody |
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| MBS175164-5x01mg | MyBiosource | 5x0.1mg | 1870 EUR |
Rapid Rat Antibody Isotyping Kit, 5pk |
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| 113103 | Antibody Research Corporation | 5 PKS | Ask for price |
Rapid Human Antibody Isotyping Kit, 5pk |
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| 113101 | Antibody Research Corporation | 5 PKS | Ask for price |
Rapid Mouse Antibody Isotyping Kit, 5pk |
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| 113102 | Antibody Research Corporation | 5 PKS | Ask for price |
Anti-GST3/GST pi/GSTP1 Antibody |
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| PA1590 | BosterBio | 100ug/vial | 154 EUR |
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Description: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat_x000D_;Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse |
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Anti-Plant GST Rabbit antibody |
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| STJ97094 | St John's Laboratory | 200 µl | 236.4 EUR |
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Description: Rabbit polyclonal to Plant GST Rabbit. |
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Mouse Anti Gst Monoclonal Antibody |
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| DMABT-54781MG | Creative Diagnostics | 2 ml | 525 EUR |
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Description: Mouse |
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Mouse anti GST Monoclonal Antibody |
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| MBS460277-01mg | MyBiosource | 0.1mg | 320 EUR |
Mouse anti GST Monoclonal Antibody |
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| MBS460277-5x01mg | MyBiosource | 5x0.1mg | 1390 EUR |
Mouse anti GST Monoclonal Antibody |
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| TA354391 | Origene Technologies GmbH | 100 µg | Ask for price |
Rabbit anti GST Polyclonal Antibody |
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| MBS460211-01mg | MyBiosource | 0.1mg | 320 EUR |
Rabbit anti GST Polyclonal Antibody |
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| MBS460211-5x01mg | MyBiosource | 5x0.1mg | 1390 EUR |
Rabbit anti GST Polyclonal Antibody |
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| TA354318 | Origene Technologies GmbH | 100 µg | Ask for price |
GST Antibody |
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| 49060 | SAB | 100ul | 499 EUR |
GST Antibody |
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| 49060-100ul | SAB | 100ul | 399.6 EUR |
GST Antibody |
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| 49060-50ul | SAB | 50ul | 286.8 EUR |
GST Antibody |
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| 48048 | SAB | 100ul | 429 EUR |
GST Antibody |
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| 48048-100ul | SAB | 100ul | 399.6 EUR |
GST Antibody |
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| 48048-50ul | SAB | 50ul | 286.8 EUR |
GST Antibody |
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| abx159522-100g | Abbexa | 100 µg | Ask for price |
GST Antibody |
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| 20-abx159522 | Abbexa |
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GST Antibody |
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| abx159522-10g | Abbexa | 10 µg | 200 EUR |
GST Antibody |
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| abx159522-50g | Abbexa | 50 µg | 250 EUR |
GST Antibody |
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| abx159523-100g | Abbexa | 100 µg | Ask for price |
GST Antibody |
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| 20-abx159523 | Abbexa |
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GST Antibody |
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| abx159523-10g | Abbexa | 10 µg | 187.5 EUR |
GST Antibody |
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| abx159523-50g | Abbexa | 50 µg | 250 EUR |
GST Antibody |
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| abx333606-100g | Abbexa | 100 µg | Ask for price |
GST Antibody |
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| abx333606-100ul | Abbexa | 100 ul | 594 EUR |
GST Antibody |
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| abx333606-20g | Abbexa | 20 µg | 437.5 EUR |
GST Antibody |
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| abx333606-50g | Abbexa | 50 µg | Ask for price |
GST Antibody |
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| CSB-PA847596ZA01PLO-02mg | Cusabio | 0.2mg | Ask for price |
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Description: Recombinant Plasmodium falciparum GST protein |
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GST Antibody |
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| CSB-PA847596ZA01PLO-10mg | Cusabio | 10mg | Ask for price |
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Description: Recombinant Plasmodium falciparum GST protein |
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GST Antibody |
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| CSB-PA187265- | Cusabio | each | 402 EUR |
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Description: A polyclonal antibody against GST. Recognizes GST from . This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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GST Antibody |
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| CSB-PA187265-100ul | Cusabio | 100ul | 454.8 EUR |
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Description: A polyclonal antibody against GST. Recognizes GST from . This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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GST Antibody |
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| abx448858-100ug | Abbexa | 100 ug | 661.2 EUR |
GST, Antibody |
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| GWB-917A67 | GenWay Biotech | 1 ml | Ask for price |
GST Antibody |
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| GWB-AD0FE0 | GenWay Biotech | 0.5 mg | Ask for price |
GST Antibody |
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| GWB-B99316 | GenWay Biotech | 0.25 mg | Ask for price |
GST Antibody |
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| GWB-4E789C | GenWay Biotech | 1 ml | Ask for price |
GST Antibody |
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| GWB-57EF67 | GenWay Biotech | 0.1 mg | Ask for price |
GST Antibody |
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| GWB-3532C4 | GenWay Biotech | 0.1 mg | Ask for price |
GST Antibody |
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| GWB-377968 | GenWay Biotech | 0.25 mg | Ask for price |
GST Antibody |
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| GWB-1C1FB3 | GenWay Biotech | 2 ml | Ask for price |
GST Antibody |
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| E10-20033 | EnoGene | 100μg/100μl | 225 EUR |
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Description: Available in various conjugation types. |
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GST Antibody |
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| E300003a | EnoGene | 100ug/200ul | 295 EUR |
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Description: Available in various conjugation types. |
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GST Antibody |
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| E8M0807-1 | EnoGene | 100ul | 225 EUR |
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Description: Available in various conjugation types. |
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GST Antibody |
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| E8M0807-12 | EnoGene | 100ul | 225 EUR |
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Description: Available in various conjugation types. |
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GST Antibody |
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| E81003-8 | EnoGene | 100ul | 225 EUR |
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Description: Available in various conjugation types. |
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GST antibody |
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| MBS200015-005mL | MyBiosource | 0.05mL | 260 EUR |
GST antibody |
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| MBS200015-01mL | MyBiosource | 0.1mL | 345 EUR |
GST antibody |
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| MBS200015-5x01mL | MyBiosource | 5x0.1mL | 1300 EUR |
GST Antibody |
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| MBS5312553-01mg | MyBiosource | 0.1mg | 975 EUR |
GST Antibody |
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| MBS5312553-5x01mg | MyBiosource | 5x0.1mg | 4245 EUR |
GST Antibody |
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| MBS5312895-01mg | MyBiosource | 0.1mg | 975 EUR |
GST Antibody |
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| MBS5312895-5x01mg | MyBiosource | 5x0.1mg | 4245 EUR |
GST antibody |
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| MBS533674-01mg | MyBiosource | 0.1mg | 385 EUR |
GST antibody |
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| MBS533674-5x01mg | MyBiosource | 5x0.1mg | 1585 EUR |
GST Antibody |
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| MBS7130361-01mL | MyBiosource | 0.1mL | 325 EUR |
GST Antibody |
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| MBS7130361-5x01mL | MyBiosource | 5x0.1mL | 1455 EUR |
GST Antibody |
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| MBS850608-01mg | MyBiosource | 0.1mg | 305 EUR |
GST Antibody |
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| MBS850608-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
GST Antibody |
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| MBS850608-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
GST Antibody |
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| MBS850608-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
GST Antibody |
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| MBS850608-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
GST Antibody |
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| MBS857368-01mL | MyBiosource | 0.1mL | 310 EUR |
GST Antibody |
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| MBS857368-01mLAF350 | MyBiosource | 0.1mL(AF350) | 570 EUR |
GST Antibody |
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| MBS857368-01mLAF405 | MyBiosource | 0.1mL(AF405) | 570 EUR |
GST Antibody |
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| MBS857368-01mLBiotin | MyBiosource | 0.1mL(Biotin) | 570 EUR |
GST Antibody |
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| MBS857368-01mLFITC | MyBiosource | 0.1mL(FITC) | 570 EUR |
GST Antibody |
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| MBS857573-01mL | MyBiosource | 0.1mL | 310 EUR |
GST Antibody |
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| MBS857573-01mLAF350 | MyBiosource | 0.1mL(AF350) | 570 EUR |
GST Antibody |
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| MBS857573-01mLAF405 | MyBiosource | 0.1mL(AF405) | 570 EUR |
GST Antibody |
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| MBS857573-01mLBiotin | MyBiosource | 0.1mL(Biotin) | 570 EUR |
GST Antibody |
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| MBS857573-01mLFITC | MyBiosource | 0.1mL(FITC) | 570 EUR |
GST Antibody |
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| MBS9200245-008mL | MyBiosource | 0.08mL | 210 EUR |
GST Antibody |
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| MBS9200245-04mL | MyBiosource | 0.4mL | 430 EUR |
GST Antibody |
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| MBS9200245-5x04mL | MyBiosource | 5x0.4mL | 1910 EUR |
GST antibody |
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| MBS831604-01mg | MyBiosource | 0.1mg | 340 EUR |
GST antibody |
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| MBS831604-5x01mg | MyBiosource | 5x0.1mg | 1385 EUR |
GST antibody |
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| MBS831735-01mL | MyBiosource | 0.1mL | 300 EUR |
GST antibody |
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| MBS831735-5x01mL | MyBiosource | 5x0.1mL | 1205 EUR |
GST Antibody |
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| MBS8564740-01mL | MyBiosource | 0.1mL | 305 EUR |
GST Antibody |
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| MBS8564740-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
GST Antibody |
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| MBS8564740-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
GST Antibody |
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| MBS8564740-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
GST Antibody |
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| MBS8564740-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
GST Antibody |
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| MBS8565959-01mL | MyBiosource | 0.1mL | 305 EUR |
GST Antibody |
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| MBS8565959-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
GST Antibody |
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| MBS8565959-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
GST Antibody |
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| MBS8565959-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
GST Antibody |
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| MBS8565959-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
GST Antibody |
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| MBS850931-01mL | MyBiosource | 0.1mL | 305 EUR |
GST Antibody |
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| MBS850931-01mLAF405L | MyBiosource | 0.1mL(AF405L) | 565 EUR |
GST Antibody |
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| MBS850931-01mLAF405S | MyBiosource | 0.1mL(AF405S) | 565 EUR |
GST Antibody |
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| MBS850931-01mLAF610 | MyBiosource | 0.1mL(AF610) | 565 EUR |
GST Antibody |
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| MBS850931-01mLAF635 | MyBiosource | 0.1mL(AF635) | 565 EUR |
GST Antibody |
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| MBS9434921-005mL | MyBiosource | 0.05mL | 325 EUR |
GST Antibody |
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| MBS9434921-01mL | MyBiosource | 0.1mL | 445 EUR |
GST Antibody |
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| MBS9434921-5x01mL | MyBiosource | 5x0.1mL | 1860 EUR |
GST Antibody |
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| MBS9435840-005mL | MyBiosource | 0.05mL | 325 EUR |
GST Antibody |
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| MBS9435840-01mL | MyBiosource | 0.1mL | 435 EUR |
GST Antibody |
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| MBS9435840-5x01mL | MyBiosource | 5x0.1mL | 1810 EUR |
GST Antibody |
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| PHY5013 | PhytoAB | 150 μg | 318 EUR |
GST Antibody |
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| SMC-549D | Stressmarq | 0.1mg | 231 EUR |
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Description: Mouse Anti-Schistosoma japonicum GST Monoclonal IgG1 |
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GST Antibody |
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| SMC-549S | Stressmarq | 0.012mg | Ask for price |
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Description: Mouse Anti-Schistosoma japonicum GST Monoclonal IgG1 |
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GST antibody |
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| 10R-7762 | Fitzgerald Industries International | 500 ug | Ask for price |
GST antibody |
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| 10R-3007 | Fitzgerald Industries International | 100 ug | Ask for price |
GST antibody |
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| 10R-10442 | Fitzgerald Industries International | 100 ug | Ask for price |
GST antibody |
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| 10R-1798 | Fitzgerald Industries International | 100 ul | Ask for price |
GST antibody |
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| 10-2340 | Fitzgerald Industries International | 100 ug | Ask for price |
GST antibody |
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| 70R-13723 | Fitzgerald Industries International | 100 ug | Ask for price |
GST antibody |
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| 10R-10564 | Fitzgerald Industries International | 100 ug | Ask for price |
GST antibody |
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| 10R-2934 | Fitzgerald Industries International | 100 ug | Ask for price |
GST antibody |
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| 70R-14127 | Fitzgerald Industries International | 100 ug | Ask for price |
GST antibody |
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| 10R-1012 | Fitzgerald Industries International | 100 ul | Ask for price |
GST antibody |
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| 10R-10239 | Fitzgerald Industries International | 100 ug | Ask for price |
Anti H Pab |
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| 165603 | Antibody Research Corporation | 100 µg | Ask for price |
Anti T8 Pab |
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| 165763 | Antibody Research Corporation | 100 µg | Ask for price |
Anti KS Pab |
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| 165190 | Antibody Research Corporation | 100 µg | Ask for price |
Anti HGF Pab |
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| 165607 | Antibody Research Corporation | 100 µg | Ask for price |
Anti IL6 Pab |
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| 165622 | Antibody Research Corporation | 100 µg | Ask for price |
Anti IL8 Pab |
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| 165623 | Antibody Research Corporation | 100 µg | Ask for price |
Anti LBP Pab |
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| 165632 | Antibody Research Corporation | 100 µg | Ask for price |
Anti MIF Pab |
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| 165660 | Antibody Research Corporation | 100 µg | Ask for price |
Anti p16 Pab |
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| 165702 | Antibody Research Corporation | 100 µg | Ask for price |
Anti P40 Pab |
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| 165703 | Antibody Research Corporation | 100 µg | Ask for price |
Anti P53 Pab |
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| 165704 | Antibody Research Corporation | 100 µg | Ask for price |
CONCLUSIONS
ELISAs had larger total sensitivity than RDTs. In conjunction with different parameters, the efficiency information may also help decide which dengue diagnostics ought to be used in the course of the first few days of sickness, when the sufferers are almost certainly to current to a clinic in search of care.
Nested-PCR and a brand new ELISA-based NovaLisa check equipment for malaria prognosis in an endemic space of Thailand.
Microscopy is taken into account because the gold normal for malaria prognosis though its large software is proscribed by the requirement of extremely skilled microscopists. PCR and serological tests present environment friendly diagnostic efficiency and have been utilized for malaria prognosis and analysis.
The goal of this research was to analyze the diagnostic efficiency of nested PCR and a not too long ago developed an ELISA-based new rapid prognosis check (RDT), NovaLisa check equipment, for prognosis of malaria an infection, using microscopic technique because the gold normal. The efficiency of nested-PCR as a malaria diagnostic instrument is superb with respect to its excessive accuracy, sensitivity, specificity, and capacity to discriminate Plasmodium species.
The sensitivity and specificity of nested-PCR in contrast with the microscopic technique for detection of Plasmodium falciparum, Plasmodium vivax, and P. falciparum/P. vivax combined an infection had been 71.Four vs 100%, 100 vs 98.7%, and 100 vs 95.0%, respectively. The sensitivity and specificity of the ELISA-based NovaLisa check equipment in contrast with the microscopic technique for detection of Plasmodium genus had been 89.Zero vs 91.6%, respectively. NovaLisa check equipment supplied comparable diagnostic efficiency. Its comparatively low value, simplicity, and rapidity permits massive scale subject software.