Serum and tissue fluid samples from experimentally infected swine were tested for antibodies to Toxoplasma gondii using both an indirect ELISA and a modified agglutination test (MAT) available commercially in kit form. Ten 8-9 week-old swine were fed meatballs containing 100, 300, 500 or 1000 T. gondii oocysts and three control animals were fed meatballs with no oocysts. Post-inoculation blood samples were collected weekly until euthanasia at 35-63 days post inoculation (DPI).
Tissue fluid was obtained from diaphragm, heart and sternomastoideus muscles post-mortem. By 16 DPI, nine of 10 inoculated pigs were detected serologically using ELISA at a pre-test serum dilution of 1:50 and all ten pigs were detected by the MAT at a serum dilution of 1:25.
The last pig became positive on ELISA by 21 DPI and the 10 pigs maintained their serological status for the duration of the experiment. Heart muscle was the best overall source of tissue fluid for ELISA and all six pigs inoculated with either 500 or 1000 oocysts were positive using either diaphragm or heart tissue fluid samples.
However, 10 of 18 fluid samples from pigs receiving ≤ 300 oocysts were not detected using ELISA, including 5 of 6 from sternomastoideus muscle. The MAT used at a 1:10 pre-test dilution of tissue fluid correctly identified all 10 inoculated pigs regardless of the source muscle. Based on these data, we conclude that either assay would be useful for herd evaluation or surveillance testing using sera, and the MAT would be a good candidate assay for testing tissue fluid for the same purposes.
Nerve Terminal Staining Kit V |
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| 70034-1 | Biotium | KT | 605 EUR |
STAT Double Staining ragent for Double staining Mo |
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| NB330DB | Innovex | 25 ml | 595 EUR |
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Description: STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides |
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STAT Double Staining reagent for Double staining o |
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| NB326DB | Innovex | 25 ml | 595 EUR |
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Description: STAT Double Staining reagent for Double staining of Mouse and Rabbit antibodies, 450 plus slides |
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STAT Double Staining Reagent for Double staining o |
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| NB327DB | Innovex | 25 ml | 595 EUR |
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Description: STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides |
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PI Staining Kit |
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| abx097208-1Kit | Abbexa | 1 Kit | 276 EUR |
HE Staining Kit |
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| E-IR-R117-100mL | Elabscience Biotech | 100mL | 240 EUR |
HE Staining Kit |
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| E-IR-R117-10mL | Elabscience Biotech | 10mL | 35 EUR |
HE Staining Kit |
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| E-IR-R117-50mL | Elabscience Biotech | 50mL | 150 EUR |
HE Staining Kit |
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| E-IR-R117-each | Elabscience Biotech | each | Ask for price |
PI Staining Kit |
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| MBS826722-01mL | MyBiosource | 0.1mL | 140 EUR |
PI Staining Kit |
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| MBS826722-5x01mL | MyBiosource | 5x0.1mL | 550 EUR |
HE staining Kit |
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| MBS2567140-100mL | MyBiosource | 100mL | 255 EUR |
HE staining Kit |
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| MBS2567140-10mL | MyBiosource | 10mL | 120 EUR |
HE staining Kit |
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| MBS2567140-50mL | MyBiosource | 50mL | 195 EUR |
HE staining Kit |
|||
| MBS2567140-5x100mL | MyBiosource | 5x100mL | 1120 EUR |
DAPI Staining Kit |
|||
| abx097206-1Kit | Abbexa | 1 Kit | 276 EUR |
TRAP Staining Kit |
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| KT-008 | Kamiya Biomedical Company | 1 kit | 747 EUR |
DAPI Staining Kit |
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| abx097206-100l | Abbexa | 100 µl | 162.5 EUR |
DAPI Staining Kit |
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| abx097206-1ml | Abbexa | 1 ml | Ask for price |
DAPI Staining Kit |
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| abx097206-200l | Abbexa | 200 µl | Ask for price |
DAPI Staining Kit |
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| MBS826728-01mL | MyBiosource | 0.1mL | 140 EUR |
DAPI Staining Kit |
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| MBS826728-5x01mL | MyBiosource | 5x0.1mL | 550 EUR |
Coplin Staining Jar |
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| 08415-3 | Polysciences Europe GmbH | 3sets | 84 EUR |
Silver Staining Kit |
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| ML123-1KT | EWC Diagnostics | 1 unit | 201.16 EUR |
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Description: Silver Staining Kit |
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Cell Staining Buffer |
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| E-CK-A107-100mL | Elabscience Biotech | 100 mL | 20 EUR |
Cell Staining Buffer |
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| E-CK-A107-200mL | Elabscience Biotech | 200 mL | 35 EUR |
Cell Staining Buffer |
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| E-CK-A107-500mL | Elabscience Biotech | 500 mL | 60 EUR |
Cell Staining Buffer |
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| E16FXP005 | EnoGene | 500 ml | 40 EUR |
|
Description: Available in various conjugation types. |
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DNA Staining Reagent |
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| abx299713-100g | Abbexa | 100 µg | Ask for price |
DNA Staining Reagent |
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| abx299713-20g | Abbexa | 20 µg | 300 EUR |
DNA Staining Reagent |
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| abx299713-50g | Abbexa | 50 µg | Ask for price |
Cell Staining Buffer |
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| E-CK-A107-each | Elabscience Biotech | each | Ask for price |
Cell Staining Buffer |
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| MBS679239-500mL | MyBiosource | 500mL | 185 EUR |
Cell Staining Buffer |
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| MBS679239-5x500mL | MyBiosource | 5x500mL | 675 EUR |
Cell Staining Buffer |
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| MBS679240-100mL | MyBiosource | 100mL | 130 EUR |
Cell Staining Buffer |
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| MBS679240-5x100mL | MyBiosource | 5x100mL | 435 EUR |
Cell Staining Buffer |
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| MBS679241-250mL | MyBiosource | 250mL | 145 EUR |
Cell Staining Buffer |
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| MBS679241-5x250mL | MyBiosource | 5x250mL | 515 EUR |
Cell Staining Buffer |
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| MBS2567126-100mL | MyBiosource | 100mL | 90 EUR |
Cell Staining Buffer |
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| MBS2567126-200mL | MyBiosource | 200mL | 100 EUR |
Cell Staining Buffer |
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| MBS2567126-500mL | MyBiosource | 500mL | 125 EUR |
Cell Staining Buffer |
|||
| MBS2567126-5x500mL | MyBiosource | 5x500mL | 545 EUR |
DAPI Staining Solution |
|||
| MBS176580-10mL | MyBiosource | 10mL | 115 EUR |
DAPI Staining Solution |
|||
| MBS176580-50mL | MyBiosource | 50mL | 135 EUR |
DAPI Staining Solution |
|||
| MBS176580-5x50mL | MyBiosource | 5x50mL | 450 EUR |
jetBlue Protein Staining Solution |
|||
| MBS402061-20mL | MyBiosource | 20mL | 165 EUR |
jetBlue Protein Staining Solution |
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| MBS402061-500mL | MyBiosource | 500mL | 210 EUR |
Wright Staining Solution |
|||
| K1182-100 | ApexBio | 100ml | 40 EUR |
|
Description: Cell Biology|Staining Solution |
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Wright Staining Solution |
|||
| K1182-500 | ApexBio | 500ml | 64 EUR |
|
Description: Cell Biology|Staining Solution |
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ELVIS ID Staining Kit |
|||
| SK-ELVIS-100 | Quidel | 100 Tests | Ask for price |
ELVIS ID Staining Kit |
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| SK-ELVIS-1000 | Quidel | 1000 Tests | Ask for price |
ELVIS ID Staining Kit |
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| SK-ELVIS-200 | Quidel | 200 Tests | Ask for price |
ELVIS ID Staining Kit |
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| SK-ELVIS-500 | Quidel | 500 Tests | Ask for price |
Colloidal Protein Staining Solution |
|||
| 51581 | Sisco Laboratories | 100 ml | 25.5 EUR |
|
Description: Part B |
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Hoechst 33342 Staining Kit |
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| abx097207-1Kit | Abbexa | 1 Kit | 276 EUR |
Hoechst 33342 Staining Kit |
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| abx097207-100l | Abbexa | 100 µl | 162.5 EUR |
Hoechst 33342 Staining Kit |
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| abx097207-1ml | Abbexa | 1 ml | Ask for price |
Hoechst 33342 Staining Kit |
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| abx097207-200l | Abbexa | 200 µl | Ask for price |
Hoechst 33342 Staining Kit |
|||
| MBS826727-01mL | MyBiosource | 0.1mL | 140 EUR |
Hoechst 33342 Staining Kit |
|||
| MBS826727-5x01mL | MyBiosource | 5x0.1mL | 550 EUR |
Leishman Staining Solution |
|||
| 68015 | Sisco Laboratories | 250 ml | 2.05 EUR |
|
Description: Part A |
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DNA STAINING AGENT (1ML) |
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| NOVELJUICE | Consort | each | 80 EUR |
Hematoxylin Staining Buffer |
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| E-IR-R120-15mL | Elabscience Biotech | 15mL | 60 EUR |
Hematoxylin Staining Buffer |
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| E-IR-R120-50mL | Elabscience Biotech | 50mL | 170 EUR |
Hematoxylin Staining Buffer |
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| E-IR-R120-5mL | Elabscience Biotech | 5mL | 25 EUR |
Hematoxylin Staining Buffer |
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| E-IR-R120-each | Elabscience Biotech | each | Ask for price |
Hematoxylin staining Buffer |
|||
| MBS2567138-15mL | MyBiosource | 15mL | 125 EUR |
Hematoxylin staining Buffer |
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| MBS2567138-50mL | MyBiosource | 50mL | 190 EUR |
Hematoxylin staining Buffer |
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| MBS2567138-5mL | MyBiosource | 5mL | 95 EUR |
Hematoxylin staining Buffer |
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| MBS2567138-5x50mL | MyBiosource | 5x50mL | 815 EUR |
Cartilage Staining Solution |
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| MBS355813-100mL | MyBiosource | 100mL | 185 EUR |
Cartilage Staining Solution |
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| MBS355813-5x100mL | MyBiosource | 5x100mL | 530 EUR |
Cell Cycle Staining Kit |
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| KTA2020-each | Abbkine | each | Ask for price |
Cell Cycle Staining Kit |
|||
| KTA2020-100T | Abbkine | 100 T | 119 EUR |
|
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. |
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Cell Cycle Staining Kit |
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| KTA2020-50T | Abbkine | 50 T | 69 EUR |
|
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. |
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Cell Cycle Staining Kit |
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| MBS9718793-100Tests | MyBiosource | 100Tests | 225 EUR |
Cell Cycle Staining Kit |
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| MBS9718793-500Tests | MyBiosource | 500Tests | 355 EUR |
Cell Cycle Staining Kit |
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| MBS9718793-50Tests | MyBiosource | 50Tests | 195 EUR |
Cell Cycle Staining Kit |
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| MBS9718793-5x500Tests | MyBiosource | 5x500Tests | 1580 EUR |
HISTOCHEMISTRY STAINING TRAY |
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| ATC-12 | IBI Scientific | 12 PLACE - CLEAR LID | 226.1 EUR |
HISTOCHEMISTRY STAINING TRAY |
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| ATC-24 | IBI Scientific | 24 PLACE - CLEAR LID | 342.21 EUR |
HISTOCHEMISTRY STAINING TRAY |
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| AT-12 | IBI Scientific | 12 PLACE - BLACK LID | 226.1 EUR |
HISTOCHEMISTRY STAINING TRAY |
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| AT-24 | IBI Scientific | 24 PLACE - BLACK LID | 342.21 EUR |
Exosome fluorescent staining |
|||
| S104 | 101Bio | - | 800 EUR |
PI / RNase Staining Buffer |
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| 20-abx090619 | Abbexa |
|
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Live NucRed staining kit |
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| EGY069 | EnoGene | >100T | 210 EUR |
PI / RNase Staining Buffer |
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| abx090619-100l | Abbexa | 100 µl | 162.5 EUR |
PI / RNase Staining Buffer |
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| abx090619-1ml | Abbexa | 1 ml | Ask for price |
PI / RNase Staining Buffer |
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| abx090619-200l | Abbexa | 200 µl | 200 EUR |
Live NucRed staining kit |
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| MBS8584964-100Tests | MyBiosource | 100Tests | 310 EUR |
Live NucRed staining kit |
|||
| MBS8584964-5x100Tests | MyBiosource | 5x100Tests | 1405 EUR |
PI / RNase Staining Buffer |
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| MBS355225-100Tests | MyBiosource | 100Tests | 255 EUR |
PI / RNase Staining Buffer |
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| MBS355225-50Tests | MyBiosource | 50Tests | 215 EUR |
PI / RNase Staining Buffer |
|||
| MBS355225-5x100Tests | MyBiosource | 5x100Tests | 845 EUR |
ProsiBlue Gel Staining Solution |
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| P7300-050 | GenDepot | 500ml | 208.8 EUR |
Stains All Gel Staining Kit |
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| KT-792 | Kamiya Biomedical Company | 20 tests | 737 EUR |
7-AAD Staining Solution |
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| MBS669126-200Tests | MyBiosource | 200Tests | 145 EUR |
7-AAD Staining Solution |
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| MBS669126-5x200Tests | MyBiosource | 5x200Tests | 655 EUR |
Ponceau S Protein Staining Solution |
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| PS003-B500ML | Bio-Helix | 500 ml | 25 EUR |
Live NucGreen staining kit |
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| EGY068 | EnoGene | >100T | 210 EUR |
Dead NucGreen staining kit |
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| EGY070 | EnoGene | >100T | 210 EUR |
Live NucGreen staining kit |
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| MBS8584963-100Tests | MyBiosource | 100Tests | 310 EUR |
Live NucGreen staining kit |
|||
| MBS8584963-5x100Tests | MyBiosource | 5x100Tests | 1405 EUR |
Dead NucGreen staining kit |
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| MBS8584965-100Tests | MyBiosource | 100Tests | 310 EUR |
Dead NucGreen staining kit |
|||
| MBS8584965-5x100Tests | MyBiosource | 5x100Tests | 1405 EUR |
GEL STAINING/INCUBATION BOX |
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| SB-10 | IBI Scientific | LIGHT SHIELDING | 94.44 EUR |
QUANTOMTx IQ/OQ Protocol |
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| LBQ14001 | Westburg | each | 991.9 EUR |
Ponceau S Staining Solution |
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| 41640009-1 | Glycomatrix | 500 mL | 40.13 EUR |
Ponceau S Staining Solution |
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| 41640009-2 | Glycomatrix | 1 L | 58.16 EUR |
Ponceau S Staining Solution |
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| B7778-100000 | ApexBio | 100 g | 78 EUR |
|
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes |
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Ponceau S Staining Solution |
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| B7778-50000 | ApexBio | 50 g | 53 EUR |
|
Description: Rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes |
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Ponceau S Staining Solution |
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| 14092 | Sisco Laboratories | 100 ml | 13.13 EUR |
|
Description: Part B |
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Ponceau S Staining Solution |
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| EZWB08-1x-100mL | Shanghai WSHT Biotechnology | 100mL | 9.6 EUR |
Ponceau S Staining Solution |
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| EZWB08-1x-500mL | Shanghai WSHT Biotechnology | 500mL | 32.4 EUR |
Giemsa Staining Solution (10X) |
|||
| K1181-100 | ApexBio | 100ml | 40 EUR |
|
Description: Cell Biology|Staining Solution |
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Giemsa Staining Solution (10X) |
|||
| K1181-500 | ApexBio | 500ml | 64 EUR |
|
Description: Cell Biology|Staining Solution |
|||
SDS-PAGE Staining Solution |
|||
| GR103068 | Genorise Scientific | 100 mL | 58 EUR |
Ponceau S Staining Solution |
|||
| PW005 | Bio Basic | 100ml | 82.97 EUR |
Ponceau S Staining Solution |
|||
| MBS355693-100mL | MyBiosource | 100mL | 180 EUR |
Ponceau S Staining Solution |
|||
| MBS355693-5x100mL | MyBiosource | 5x100mL | 500 EUR |
Sirius Red Staining Solution |
|||
| MBS355815-100mL | MyBiosource | 100mL | 195 EUR |
Sirius Red Staining Solution |
|||
| MBS355815-5x100mL | MyBiosource | 5x100mL | 570 EUR |
AO/PI Double Staining Kit |
|||
| K2238-100 | ApexBio | 100T | 40 EUR |
|
Description: Cell Biology|Cell Apoptosis |
|||
AO/PI Double Staining Kit |
|||
| K2238-500 | ApexBio | 500T | 120 EUR |
|
Description: Cell Biology|Cell Apoptosis |
|||
Neutral Red Staining Solution |
|||
| K1185-100 | ApexBio | 100ml | 40 EUR |
|
Description: Cell Biology|Staining Solution |
|||
EasyDip Slide Staining System |
|||
| M900-12AS-ASSORTED | ScyTek Laboratories | 1 Case(s) | 101.58 EUR |
CellSurface FLOW Staining Kit |
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| MBS668926-1Kit | MyBiosource | 1Kit | 200 EUR |
CellSurface FLOW Staining Kit |
|||
| MBS668926-5x1Kit | MyBiosource | 5x1Kit | 900 EUR |
Propidium Iodide (PI) Staining Kit |
|||
| abx097208-100l | Abbexa | 100 µl | 162.5 EUR |
Propidium Iodide (PI) Staining Kit |
|||
| abx097208-1ml | Abbexa | 1 ml | Ask for price |
Propidium Iodide (PI) Staining Kit |
|||
| abx097208-200l | Abbexa | 200 µl | Ask for price |
Dual- Color AP Staining Kit |
|||
| AP100D-1 | SBI | 100 Assays | 494.4 EUR |
Coomassie Blue Protein Staining Solution |
|||
| abx299720-100g | Abbexa | 100 µg | Ask for price |
Coomassie Blue Protein Staining Solution |
|||
| abx299720-20g | Abbexa | 20 µg | 262.5 EUR |
Coomassie Blue Protein Staining Solution |
|||
| abx299720-50g | Abbexa | 50 µg | Ask for price |
CometAssay Silver Staining Kit |
|||
| 4254-200-K | Biotechne | 200 Tests | 390.96 EUR |
PI Viability Staining Solution |
|||
| E16FXP002 | EnoGene | 150 tests | 40 EUR |
|
Description: Available in various conjugation types. |
|||
β-Galactosidase Staining Kit |
|||
| AKR-100 | Cell Biolabs | 1 kit | 345 EUR |
Methyl Green Staining Solution |
|||
| K1180-100 | ApexBio | 100ml | 40 EUR |
|
Description: Cell Biology|Staining Solution |
|||
Methyl Green Staining Solution |
|||
| abx090698-100l | Abbexa | 100 µl | 125 EUR |
Methyl Green Staining Solution |
|||
| abx090698-1ml | Abbexa | 1 ml | Ask for price |
Methyl Green Staining Solution |
|||
| abx090698-200l | Abbexa | 200 µl | 187.5 EUR |
Methyl Green Staining Solution |
|||
| MBS355816-100mL | MyBiosource | 100mL | 220 EUR |
Methyl Green Staining Solution |
|||
| MBS355816-10mL | MyBiosource | 10mL | 180 EUR |
Methyl Green Staining Solution |
|||
| MBS355816-5x100mL | MyBiosource | 5x100mL | 685 EUR |
Live/Dead Cell Staining Kit |
|||
| 55R-1434 | Fitzgerald | 100 tests | 500 EUR |
|
Description: Live/Dead Cell Staining Kit for use in the research laboratory |
|||
Red-Color AP Staining Kit |
|||
| AP100R-1 | SBI | 50 Assays | 314.4 EUR |
Beta Galactosidase Staining Kit |
|||
| 55R-1541 | Fitzgerald | 250 assays | 300 EUR |
|
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory |
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Lacto Fuchsin Staining Solution |
|||
| 97513 | Sisco Laboratories | 100 ml | 3.01 EUR |
|
Description: Part A |
|||
Blue-Color AP Staining Kit |
|||
| AP100B-1 | SBI | 50 Assays | 203 EUR |
Crystal Violet Staining Solution |
|||
| K1184-100 | ApexBio | 100ml | 40 EUR |
|
Description: Cell Biology|Staining Solution |
|||
Crystal Violet Staining Solution |
|||
| K1184-500 | ApexBio | 500ml | 64 EUR |
|
Description: Cell Biology|Staining Solution |
|||
Live-Dead Cell Staining Kit |
|||
| K2081-100 | ApexBio | 100 staining | 552 EUR |
Live-Dead Cell Staining Kit |
|||
| K2081-1000 | ApexBio | 1000T | 112 EUR |
Live-Dead Cell Staining Kit |
|||
| K2081-500 | ApexBio | 500T | 68 EUR |
Live-Dead Cell Staining Kit |
|||
| GWB-AXR262 | GenWay Biotech | 100 stainings | Ask for price |
EZ-Quick Slide Staining Set |
|||
| IW-2510 | IHC World | - | 425 EUR |
Live-Dead Cell Staining Kit |
|||
| K501-100 | Biovision | each | 561.6 EUR |
10X Ponceau S Protein Staining Solution |
|||
| PS003-L10X | Bio-Helix | 500 ml | 170 EUR |
Human Histochemistry Staining Kit |
|||
| 20-abx299039 | Abbexa |
|
|
Alkaline Phosphatase Staining Kit |
|||
| K2035-50 | Biovision | 50 assays | 454.8 EUR |
Beta-Galactosidase Staining Kit |
|||
| K2182-250 | ApexBio | 250 assays | 274 EUR |
Beta-Galactosidase Staining Kit |
|||
| K802-250 | Biovision | each | 352.8 EUR |
Beta-Galactosidase Staining Kit |
|||
| GWB-AXR372 | GenWay Biotech | 200 assays. | Ask for price |
Accuracy of a point-of-care ELISA test kit for predicting the presence of protective canine parvovirus and canine distemper virus antibody concentrations in dogs.
Canine parvovirus (CPV) and canine distemper virus (CDV) are highly infectious and often fatal diseases with worldwide distributions, and are important population management considerations in animal shelters. A point-of-care ELISA test kit is available to detect serum antibodies to CPV and CDV, and presumptively to predict protective status.
The aim of this study was to determine the diagnostic accuracy of the test compared to CPV hemagglutination inhibition titers and CDV serum neutralization titers determined by a reference laboratory, using sera collected from dogs housed at animal shelters.
The ELISA test was used under both field and laboratory conditions and duplicate specimens were processed using an extra wash step. The test kit yielded accurate results (CPV: sensitivity 92.3%, specificity 93.5%; CDV: sensitivity 75.7%, specificity 91.8%) under field conditions. CDV sensitivity was improved by performing the test under laboratory conditions and using an optical density (OD) meter (laboratory performed 94.0%; OD 88.1%). Point-of-care ELISA testing for serum CPV and CDV antibody titers was demonstrated to be a useful tool for determining antibody status when making decisions regarding the need for CPV and/or CDV vaccination and also in animal shelters for population management.